|
KMID : 1007519960050010026
|
|
Food Science and Biotechnology
1996 Volume.5 No. 1 p.26 ~ p.29
|
|
|
Factors Affecting Production of β - 1,3 - Glucanase from Bacillus sp .
|
|
Kim, Gi Nahm
Kil, Ji Oeun/Kim, Seok Hwan/Park, In Shik
|
|
|
Abstract
|
|
|
|
A bacterium capable of producing β-1,3-glucanase was isolated from soil, and identified as a strain of Bacillus. Effects of culture conditions and medium compositions on enzyme production were investigated. Among tested carbon sources, cudlan(β-1,3-glucan) was most effective for production of the enzyme, whereas mono- and di-saccharides were generally ineffective. Therefore it seemed that the enzyme was induced when β-1,3-glucan was used as a sole carbon source. The optimal concentration cudlan for the enzyme production was 7.5%. The enzyme was also induced by baker´s yeast cells as a nitrogen source, and repressed by glucose in the culture medium. The enzyme was maximally produced by cultivating the isolated Bacillus sp. at initial pH 6.0, and 32℃.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|